The PGT-A Solution employs single-cell whole-genome amplification and low-coverage whole-genome sequencing techniques to sequence 5 to 10 embryonic cells. Data analysis software statistically assesses differences in chromosome numbers to detect genomic copy number variations (CNVs). By utilizing GeneMind single-cell amplification kits and PGT-A library preparation kits, in conjunction with the GenoLab™ M / FASTASeq™ 300 / SURFSeq™ 5000 / FASTASeq™ S sequencing systems, it can precisely detect aneuploidies of 23 pairs of chromosomes and microdeletions/microduplications larger than 4 Mb across the entire genome.
Automated and simplified sequencing and data analysis workflow
Quick test from sample preparation to final report within 11 hours
Compatible with commonly used NGS library preparation kits and bioinformatics tools
Accurately detects chromosomal aneuploidy and CNVs from low-input DNA (e.g., single cell)
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Parameter |
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Sample volume |
5-10 embryo cells |
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Library preparation |
WGA |
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Method |
Whole genome sequencing |
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Read length |
SE75 for GenoLab M and FASTSeq 300，SE50 for SURFSeq 5000 |
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Sequencing platform |
GenoLab M |
FASTAseq 300 |
SURFSeq 5000 |
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No. of samples/run |
1 FCM : 32 2 FCM/1 FCH : 64 1 FCM+1 FCH: 96 2 FCH: 128 |
1 FCM : 12 1 FCH : 32 |
1 FCH : 160 2 FCH : 320 |
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Turn-around-time |
24 h |
16 h |
23 h |
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reads requirement/sample |
GenoLab M/FASTASeq 300: Unique reads per sample ≥ 3.5M SURFSeq 5000: Unique reads per sample ≥ 4M |
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Report generation |
Local analysis and report system |
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