Dual flow cell to support 5 different modes/run
Compatible with most common used applications
FCH PE150≤24h
Flexible FQ upload mode, no need to wait for one flow cell finish the run
Balance mode: Q30≥90%，Q40≥85%
Enhanced mode: Q50＞70%， Q40＞95%， Q30＞97%
Wide range selection for sample volume in one run
Most cost-effective for each sample
| SURFSeq 5000 V2.0 Sequencing Kits | |||||||
| Flow Cell | Lane | Throughput（Reads/FC） | Type | Read Length | Data output1 (FC×2) | Q value1(Enhanced mode) | TAT2(h)/FC |
| FCM | 4 | 500 M | 100 cycles | SE100 | 50 Gb×2 |
Q30≥95% Q40≥90% |
12 hr |
| PE50 | 50 Gb×2 | 12 hr | |||||
| 200 cycles | PE100 | 100 Gb×2 | 18 hr | ||||
| 300 cycles | PE150 | 150 Gb×2 | 22 hr | ||||
| 600 cycles | PE300 | 300 Gb ×2 | 48 hr | ||||
| 800 cycles | PE400 | 400 Gb×2 |
Q30≥85% Q40≥80% |
68 hr3 | |||
| FCH | 4 | 2000 M | 50cycles | SE50 | 100 Gb×2 |
Q30≥95% Q40≥90% |
14 hr |
| 100cycles | SE100 | 200 Gb×2 | 21 hr | ||||
| PE50 | 200 Gb×2 | 21 hr | |||||
| 200cycles | PE100 | 400 Gb×2 | 26 hr | ||||
| 300cycles | PE150 | 600 Gb×2 | 30 hr | ||||
| FCP | 4 | 3600 M | 50 cycles | SE50 | 180 Gb×2 | 16 hr | |
| 100 cycles | SE100 | 360 Gb×2 | 24 hr | ||||
| 200 cycles | PE100 | 720 Gb×2 | 32 hr | ||||
| 300 cycles | PE150 | 1.1 Tb×2 | 38 hr | ||||
1. This parameter is obtained based on the average of multiple test resuts of GeneMind P3 standard library. The data output and the proportion of high-quality data are affected by factors such as sample type, sample quality and effective flow cell utilization. The actual performance may vary.
2. The TAT (tumaround time)for a standard run (paired-end 8+8 indexing)encompasses the entire process from library loading to the generationtion of base-called sequence files.
3. PE400 sequencing requires two kits with manual kit replacement in between, the time excludes manual replacement time.
| SURFSeq 5000 CMS V1.0 Sequencing Kits1 | |||||||||
| Flow Cell | Lane | Throughput（Reads/FC） | Type | Read Length |
Dataoutput2 (FC×2) |
Q302 |
Q402 |
Q502 |
TAT3(h)/FC |
| FCM | 4 | 500M | 100cycles | SE100/PE50 | 50Gb×2 | Q30≥97% | Q40≥95% | Q50≥70% | 9hr |
| 200cycles | PE100 | 100Gb×2 | 12.5hr | ||||||
| 300cycles | PE150 | 150Gb×2 | 16hr | ||||||
| FCH | 4 | 2000M | 100cycles | SE100/PE50 | 200Gb×2 | 12.5hr | |||
| 200cycles | PE100 | 400Gb×2 | 18hr | ||||||
| 300cycles | PE150 | 600Gb×2 | 24hr | ||||||
| FCP | 4 | 3600M | 100cycles | SE100 | 360Gb×2 | 14hr | |||
| 200cycles | PE100 | 720Gb×2 | 21.5hr | ||||||
| 300cycles | PE150 | 1.1Tb×2 | 29hr | ||||||
1. This parameter is obtained based on the average of multiple test resuts of GeneMind P3 standard library. The data output and the proportion of high-quality data are affected by factors such as sample type, sample quality and effective flow cell utilization. The actual performance may vary.
2. The TAT (tumaround time)for a standard run (paired-end 8+8 indexing)encompasses the entire process from library loading to the generationtion of base-called sequence files.
| Applications | Read Length | Data/sample | Number of samples/FC | ||
| FCM-500M | FCH-2000M | FCP-36000M | |||
| Oncology Panel (580genes)-Tissue | PE150 | 6 Gb | 16 | 96 | 164 |
| Oncology Panel (580genes)-Plasma | PE150 | 50 Gb | 2 | 8 | 16 |
| NIPT Plus | SE50 | 17M reads | / | 96 | 180 |
| NIPT Pro | SE50 | 30M reads | / | 64 | 120 |
| TB-tNGS | SE100 | 2M reads | 192 | 768 | / |
| PGT-A | SE100 | 5M Reads | / | 160 | 280 |
| cWES (100X) | PE150 | 6 Gb | 24 | 96 | 168 |
| WES (100X) | PE150 | 12 Gb | 12 | 48 | 84 |
| WGS (30X） | PE150 | 110 Gb | 1 | 4 | 8 |
| Single cell 3' RNA-seq | PE150 | 120 Gb | 1 | 4 | 6 |
The above sample numbers for different applications are only for reference. User need to adjust the sample numbers according to the actual experiment.
| Specification | |
| Dimensions | 1090mm(L)×690mm(W)×810mm(H) |
| Weight | 240kg |
| Operating Environment | Temperature:19℃-25℃ |
| Humidity:20%-80% relative humidity, non-condensing | |
| Altitude: below 300m | |
| Instrument Control Computer | Operating System:Windows 10 x64 |
| CPU: Intel Xeon Gold 6342 | |
| Memory:256GB | |
| Hard Drive1: 2TB SSD | |
| Hard Drive2: 17TB HDD | |
| SURFSeq 5000 V2.0 | ||
| Catalog Number | Product name | Specification |
| SQ00025 | SURFSeq 5000 Sequencing System Set | RUO |
| S000351 | SURFSeq 5000 Sequencing Kit V2.0 （FCM 100cycles） | FCM 100cycles |
| S000356 | SURFSeq 5000 Sequencing Kit V2.0 （FCM 200cycles） | FCM 200cycles |
| S000358 | SURFSeq 5000 Sequencing Kit V2.0 （FCM 300cycles） | FCM 300cycles |
| S000272 | SURFSeq 5000 Sequencing Kit V2.0 （FCM 600cycles） | FCM 600cycles |
| S000590 | SURFSeq 5000 Sequencing Kit V2.0 (FCM PE400) | FCM PE400 |
| S000360 | SURFSeq 5000 Sequencing Kit V2.0（FCH 50cycles） | FCH 50cycles |
| S000365 | SURFSeq 5000 Sequencing Kit V2.0 （FCH 100cycles） | FCH 100cycles |
| S000367 | SURFSeq 5000 Sequencing Kit V2.0 （FCH 200cycles） | FCH 200cycles |
| S000369 | SURFSeq 5000 Sequencing Kit V2.0 （FCH 300cycles） | FCH 300cycles |
| S000371 | SURFSeq 5000 Sequencing Kit V2.0（FCP 50cycles） | FCP 50cycles |
| S000373 | SURFSeq 5000 Sequencing Kit V2.0 （FCP 100cycles） | FCP 100cycles |
| S000375 | SURFSeq 5000 Sequencing Kit V2.0 （FCP 200cycles） | FCP 200cycles |
| S000377 | SURFSeq 5000 Sequencing Kit V2.0 （FCP 300cycles） | FCP 300cycles |
| SURFSeq 5000 CMS V1.0 | ||
| Cat. No. | Product name | Specification |
| S000547 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCM 100cycles） | CMS FCM 100cycles |
| S000552 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCM 200cycles） | CMS FCM 200cycles |
| S000554 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCM 300cycles） | CMS FCM 300cycles |
| S000556 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCH 100cycles） | CMS FCH 100cycles |
| S000559 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCH 200cycles） | CMS FCH 200cycles |
| S000561 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCH 300cycles） | CMS FCH 300cycles |
| S000563 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCP 100cycles） | CMS FCP 100cycles |
| S000566 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCP 200cycles） | CMS FCP 200cycles |
| S000568 | SURFSeq 5000 CMS Sequencing Kit V1.0 （FCP 300cycles） |
CMS FCP 300cycles |
For Research Use Only. Not intended for use in diagnostic or clinical procedures. Not available in all countries.
