HLA Typing (11 Loci) Solution


The 11 Loci HLA Solution is a next-generation sequencing (NGS)-based genotyping kit designed to deliver high-resolution, full-length HLA typing across 11 key loci. Leveraging probes based on the IMGT/HLA Database v3.50, this kit covers a genomic region of approximately 130 kb, including UTRs, exons, and introns. With a flexible workflow that accommodates both manual and automated library preparation, the kit provides a scalable, high-throughput solution suitable for laboratories with varying sample sizes.

* For Research Use Only. Not intended for use in diagnostic or clinical procedures. Not available in all countries.
Features
  • High Resolution

    Supports 4-field (8-digit) high-resolution genotyping, enabling precise allele identification

  • Full-Length Coverage

    Covers full-length Covers full-length sequence including UTRs, exons, and introns (excluding highly repetitive elements), spanning approximately 130 kb of genomic region

  • Comprehensive Loci

    Covers HLA-A, B, C, DPA1, DPB1, DQA1, DQB1, and DRB1/3/4/5, ensuring complete allele coverage across all major HLA loci

  • Automated Data Analysis & Intelligent Reporting

    Built-in dedicated HLA analysis software automatically delivers 8-digit genotyping results with full annotation, reference

HLA Typing Workflow






Parameters

Product Name

Human Leukocyte Antigen (HLA) Genotyping Kit (11 Loci)

Targeted Genes

Class I:HLA-A, B, C; Class II:HLA-DPA1, DPB1, DQA1, DQB1, DRB1/3/4/5; 

Full-length sequences of 11 loci

Specifications

48 tests/kit ; 96 tests/kit

Hybridization

 3h rapid hybridization supported; maximum 12-plex pooling

Sample Type

Whole Blood

Sample Requirements

Collected in EDTA tubes; ≥ 2 mL blood

Genotyping Resolution

Field 4 / Digit 8

Data Output per Sample

0.3Gb (PE150)

Sequencing Type

PE150

Sequencing Platform

FASTASeqTM 300

GenoLabTM  M

FASTASeqTM  S

SURFSeqTM  5000

Throughput per Run

1 FCM:100

1 FCH:224

1 FCP:400

1 FCM:196

1 FCH:400

1 FCL:16

1 FCM:32

1 FCM:600

1 FCH:2400

Turnaround time

13-24h

  38-50h

 

6.8-7.5h

16-31h

DNA Input Requirement

Minimum 30 ng DNA input; 100 ng DNA recommended

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