Targeted Next-generation Sequencing (tNGS) for Pathogenic Microorganism combines multiplex PCR and NGS to identify hundreds of known pathogenic microorganisms and their associated virulence and/or drug resistance genes in the specimens. The method excels in its sensitivity, enabling the detection of pathogens even at low concentrations in a cost-effective manner.
Based on super-multiplex targeted amplification and NGS technology, GeneMind Pathogen Solution (GPS) can be used for the detection of pathogens in general scenarios, including 476 pathogens, 192 drug resistance genes/mutations and 25 hypervirulence genes, as well as co-detection of DNA and RNA Pathogens. It is suitable for various clinical scenarios, such as moderate or severe infections, mixed infections, and suspected drug resistant infections.
476 pathogens, completely covering general clinicalscenarios including respiratory system, centralnervous system, and focal infections.
Drug resistance genes and hypervirulence genesdetection.
In-house super-multiplex targeted amplification andprimer-dimer removal technology, achieving >90%effective data rate.
Expanded targets still with superb performance, with LoD as low as 50 copies/mL.
Single-tube amplification system simplifies experimental operation with a fast TAT time of 10.5 hours for thewhole workflow.
DNA & RNA pathogen Co-detection.
Internal control and in-house tNGS quantitativealgorithm.
Correction of PCR amplification bias to achieveaccurate pathogen quantification.
|
PARAMETERS |
||
|
Sample type |
BALF ≥5 mL, Sputum ≥2 ml, Nasopharyngeal swabs ≥2; CSF ≥2 mL; Peripheral blood: Adult ≥5 mL/Child ≥2 mL, Urine ≥5 mL, Vaginal secretion swab ≥2; Hydrothoraxor ascite ≥5 mL; Pus or joint aspirate ≥2 ml;Puncture specimen / Tissue 0.2-2 g |
|
|
Method |
multiplex PCR and NGS |
|
|
Read length |
SE50 |
|
|
Sequencing platform |
FASTASeqTM S |
FASTASeqTM 300 |
|
Number of samples/run |
FCL: 24 |
FCL: 50 FCM: 125 FCH: 280 FCP: 500
|
|
Turnaround time (Hr) |
10.5-11.5 |
13-16 |
|
Limit of detection (LoD) |
50 copies/mL |
|
|
Reads requirement/sample |
>0.5 M |
|
|
Report generation |
Local analysis and report system |
|
