Human pancreatic islet-derived stromal cells reveal combined features of mesenchymal stromal cells and pancreatic stellate cells

The study isolated human pancreatic islet-derived mesenchymal stem cells (hPD-MSCs) from donor islets using lentiviral transduction of human telomerase reverse transcriptase. These cells were subsequently identified as adipogenic and osteogenic stem cells through immunostaining, flow cytometry, and differentiation potential assays. Based on transcriptomic sequencing results from the GeneMind GenoLab M sequencing platform, the research team compared the gene expression profiles of hPD-MSCs with those of other mesenchymal cells. They found that hPD-MSCs exhibited typical characteristics of mesenchymal stem cells (morphological features and surface marker expression) and possessed the ability to differentiate into adipogenic and osteogenic lineages. Additionally, they identified specific genes that distinguish pancreatic mesenchymal stem cells from mesenchymal stem cells of other origins, including ISL1, which is related to pancreatic function, and NPT, which is associated with neural development.

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