m6A is an RNA modification that is abundant in mammalian cells and catalyzed by the methyltransferase complex (MTC), of which the METTL3/METTL14 heterodimer is the catalytic subunit of MTC. Studies have shown that m6A methylation plays an important role in a variety of physiological and pathological processes by regulating RNA transcription, splicing, stability and translation. MTC can enrich on H3K27ac chromatin to promote the m6A modification of eRNA and paRNA, thereby affecting gene transcription
In this study，a genome-wide CRISPR/Cas9 screen combined with the TETon-BIFC reporter system systematically identified a series of regulators of METTL3/METTL14 heterodimer depolymerization in A549 cells. Researchers also performed CUT&Tag sequencing of wild-type METTL3 and METTL3-3KR mutant A549 cells by using GeneMind SURFSeq 5000 high throughput sequencing platform to verify the inhibitor effect of METTL3 acetylation on H3K27ac chromatin localization at the genomic level.
