Improving Seed Shattering Resistance in Wild O. alta Rice with Mesoporous Silica Nanoparticle Delivery Systems

To address food security challenges and climate change, the polyploid wild rice Oryza alta has been explored as a potential crop, although it suffers from seed shattering. We employed mesoporous silica nanoparticles(MSNs) to deliver small interfering RNAs(siRNAs) for targeted gene silencing. Through different methods to successfully build MSN-siRNA system, and then tracking MSN in rice leaves by TEM, O. alta treated with MSN-siRNA via spray or infiltration, histological analysis, fluorescence microscopy, BTS experiment, RNA extraction, RT-PCR and quantitative PCR, betacyanin extraction and detection, transcriptome sequencing (GeneMind SURFSeq 5000 platform, PE150). The results showing our MSN-siRNA system provides a fexible, non permanent approach to modifying croptraits, ofering a promising tool for sustainable agricultural practices.

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