Nano-bio interaction of magnetic nanoparticles with cells in a tumor at the single-cell level

Magnetic nanoparticles (MNPs), due to their magnetic properties, can be activated by alternating magnetic field heating or unheated low frequency magnetic fields, thereby exerting intrinsic therapeutic effects or promoting drug release, while the accumulation of MNPs at specific sites can be monitored by magnetic resonance imaging or magnetic particle imaging.By studying the penetration and distribution of unmodified pegylated ferric oxide magnetic nanoparticles (MNPs) or pegylated ferric oxide MNPs (ligands for intelligent targeting of acidic tumor microenvironments) modified by low pH insertion peptide (pHLIP) in mouse models of breast cancer, we found that MNPs entered tumor tissues through vascular rupture and endothymal cell endocytosis. Subsequently, single-cell RNA sequencing based on GenoLab M high throughput sequencer identified the cell populations that take up MNPs in tumor tissues and revealed the accumulation bias of MNPs in regulatory Trem2+ tumor-associated macrophages.

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