3D Spheroid Configurations Are Possible Indictors forEvaluating the Pathophysiology of Melanoma Cell Lines

To study the molecular mechanisms responsible for inducing the spatial proliferation of malignant melanomas (MM), three-dimension (3D) spheroids were produced from several MM cell lines , and their 3D architectures and cellular metabolisms were evaluated by phase-contrast microscopy and Seahorse bio-analyzer, respectively. In addition, among these five cell lines, we selected two distinct MM cells, one of which was horizontally circular shaped and the other non-horizontally circular shaped and subjected them to RNA sequence analysis by GenoLab M platform to elucidate possible master regulatory genes responsible for these structural diversities. Our findings suggest that 3D spheroid configuration has the potential for serving as an indicator of the pathophysiological activities associated with MM.

Read           Download