In this study, we measured cytotoxicity by several anti-tumor agents, cellular metabolism including mitochondrial and glycolytic functions by a Seahorse bio-analyzer, the physical properties, size and stiffness of the 3D spheroids, trypsin sensitivities. Moreover, GenoLab M high-throughput sequencer was used for RNA sequencing analysis to elucidate possible biological signaling mechanisms responsible for causing such aberrantly expressed Rec induced effects.
The findings reported here indicate that the aberrantly expressed Rec-induced modulation of the cell viability and drug sensitivity may be GPCR mediated. The findings reported here indicate that the aberrantly expressed Rec-induced modulation of the cell viability and drug sensitivity may be GPCR mediated.
