FASTASeq 300* High-throughput Sequencing Platform
FASTASeq 300* is a desktop high-throughput sequencing platform that focuses on targeted sequencing and whole-genome low-pass sequencing. Its innovative breakthroughs in sequencing chemistry, high-density flow cell, fluid design and base identification algorithms bring users more flexibility, more consistent data quality and faster delivery of high-quality data.
*Unless otherwise informed, GeneMind sequencing platform and related sequencing reagents are not available in the USA, Canada, Australia, Japan, Singapore, Western Europe, Southern Europe, and Nordic countries yet.
Flexible
-Support two types of flow cells and multiple read lengths
-Multi-time output (MTO)
-Libraries can be loaded on each flow cell lane automatically or manually
Easy-to-use
-Pre-configured, plug-and-play cartridges
-Support RFID reader module
-Libraries can be directly used for sequencing after preparation
Rapid
-mNGS (SE50 4.5hrs)
-Targeted sequencing (PE75 11.5hrs)
-Targeted sequencing (PE150 19.5hrs)
| Flowcell | Flowcell Type | Lane | Reads | Read Length | Output | Q30 | index(0) Sequecing Time(h) | index(8+8) Sequecing Time(h) |
| Single | FCM | 4 | 100M | SE50 | 5Gb | 85% | 4.5 | 5.5 |
| SE75 | 7.5Gb | 85% | 6 | 7 | ||||
| PE75 | 15Gb | 85% | 11.5 | 13 | ||||
| PE150 | 30Gb | 85% | 19.5 | 21 | ||||
| FCH | 4 | 250M | SE50 | 12.5Gb | 85% | 5 | 6.5 | |
| SE75 | 18.5Gb | 85% | 6.5 | 8 | ||||
| PE75 | 37.5Gb | 85% | 12.5 | 14 | ||||
| PE150 | 75Gb | 85% | 22.5 | 24 | ||||
| FCX | 4 | 100M | PE300 | 60Gb | 85% | 45 | 48 | |
| FCL | 4 | 50M | SE100-D | 5Gb | 85% | 4.5 | 5.5 |
| Application | Data requirement | FCM Flowcell | FCH Flowcell |
| NIPT Plus | >7M unique reads/sample | 12 | 24 |
| PGT-A | >3.5M unique reads/sample | 16 | 48 |
| TB-tNGS | >2.5M raw reads/sample | 24 | 48 |
| Small tumor panel (tissue) | 0.2Gb/sample | >48 | >96 |
| Small tumor panel (ctDNA) | 5Gb/sample | 6 | 16 |
| Large tumor panel (tissue)5~10Gb/sample | 5-10Gb/sample | 6 | 16 |
| tpNGS | 0.1M-1M reads/sample | >96 | >192 |
| mNGS | 20M reads/sample | 4 | 12 |
| Dimensions | 684mm x 644mm x 615mm |
|---|---|
| Weight | 145kg |
| Operating Power | 2000VA |
| Power Requirements | 100V-240VAC,50/60Hz |
| Operating Environment | Temperature:19℃-25℃ Humidity:20%-80%(non-condensing) Altitude:0-3000m |
| Instrument Control Computer | CPU:Intel XeonSilver4126 Memory:32GB DDR4*6 Solid-state Drive:480GB Hard Drive:2TB Operating System: windows10 x64 |
| Description of Read Header | |
| Parameter | Description |
| <SN> | Serial number of the instrument. |
| <Barcodescore> | Numbers 0 to 9 are used to evaluate the sequencing quality value of the index sequence. The higher the value, the better the quality. |
| <Flowcell ID> | The ID of sequence chip. |
| <Lane> | Number of the lane, it can be L00、L01、L02、L03、L04，etc. L00 indicates that all lanes are merged. |
| <FOV> | Coordinate of the FOV. |
| <X> | X-coordinate of the cluster. |
| <Y> | Y-coordinate of the cluster. |
| <read1/2> | Read 1 or Read 2. |
| <N> | Default character. |
| <0> | Default character. |
| <Index> | The sequence of the index, if it is a combination of index1 and index2, it should be concatenated with symbol”+”. |
| Note：@<SN>:<Barcodescore>:<Flowcell ID>:<Lane>:<FOV>:<X>:<Y> <read1/2>:<N>:<0>:<Index> The header identifier is represented by the symbol “@”, while the parameter items are differentiated using the symbol “:”. |
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