Human adenoviruses (HAdVs) are common pathogens that cause respiratory infections and can also lead to various health issues such as cystitis, conjunctivitis, gastrointestinal diseases, and even encephalitis. Genomic research on human adenoviruses (HAdVs) is crucial for discovering new strains, understanding genetic variations and evolution among different genotypes, and monitoring viral evolution to prevent outbreaks. Long-read sequencing technologies (such as ONT) can overcome challenges associated with short-read sequencing, such as sequence fragmentation and complex repetitive regions, but they have higher error rates. Short-read sequencing (such as FASTASeq 300) offers high accuracy and can be used to correct long-read sequencing results.
In collaboration with the Hubei Provincial Center for Disease Control and Prevention, Genorise Biotechnology conducted sequencing and in-depth analysis of samples from 26 adenovirus-positive upper respiratory tract infection patients using the FASTASeq 300 and ONT MinION Mk1C platforms. By integrating long-read ONT sequencing with short-read FASTASeq 300 sequencing, researchers successfully assembled 32 complete HAdV genomes and conducted a comprehensive analysis. These findings provide valuable insights for HAdV epidemiological investigations and public health security.
